LOCUS       40924_23369       11906 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB585 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Gateway Binary Vectors with Organelle-Targeted Fluorescent Proteins 
            for Highly Sensitive Reporter Assay in Gene Expression Analysis of 
            Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11906)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane 
            University, Interdisciplinary Center for Science Research; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11906)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11906)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane University, 
            Interdisciplinary Center for Science Research; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2071..2091
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             2092..2802
                     /codon_start=1
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     terminator      2827..3079
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3113..3129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3137..3153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3161..3191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3206..3227)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3263..3515)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3669..4691)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4743..4922)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5428..5452)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5973..6761
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7010..7598
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7784..7924)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8268..8462)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8531..9601)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10033..10659)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggct ccaaagaaga agagaaaggt catggtgtct
     2101 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     2161 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     2221 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     2281 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc
     2341 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     2401 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     2461 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     2521 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaagcgac
     2581 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     2641 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     2701 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     2761 agatactgcg acctccctag caaactgggg cacaaactta attgagctta gagctcgaat
     2821 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     2881 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2941 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     3001 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     3061 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     3121 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3181 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     3241 gcgcctattt ctattaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
     3301 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     3361 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     3421 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     3481 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcggggg atcgatcccg
     3541 cgaactgtgg acgagaactg tgccaccaag cgtaaggccg ttctctcgca tttgccttgc
     3601 taggctcgcg cgagttgctg gctgaggcgt tctcgaaatc agctcttgtt cggtcggcat
     3661 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     3721 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     3781 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     3841 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     3901 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     3961 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     4021 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     4081 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     4141 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     4201 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     4261 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     4321 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
     4381 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
     4441 ctgaatgccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     4501 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     4561 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     4621 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     4681 ggctttttca tatcttattg ccccccggga tcagatctgg attgagagtg aatatgagac
     4741 tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata
     4801 tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta
     4861 tgtgcttagc tcattaaact ccagaaaccc gcggctgagt ggctccttca acgttgcggt
     4921 tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga
     4981 ctcccttaat tctccgctca tgatcttgat cccctgcgcc atcagatcct tggcggcaag
     5041 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
     5101 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
     5161 ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
     5221 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc
     5281 ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta
     5341 atagaaataa attcagccta attcggcgtt aattcagtac attaaaaacg tccgcaatgt
     5401 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
     5461 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
     5521 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     5581 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     5641 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac
     5701 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     5761 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     5821 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     5881 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     5941 agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat
     6001 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     6061 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     6121 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     6181 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     6241 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     6301 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     6361 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     6421 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     6481 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     6541 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     6601 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     6661 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     6721 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt
     6781 cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac
     6841 ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat
     6901 aataagccct acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt
     6961 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7021 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7081 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7141 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7201 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7261 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7321 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7381 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7441 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7501 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7561 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7621 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7681 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7741 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     7801 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     7861 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     7921 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7981 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     8041 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
     8101 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
     8161 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
     8221 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
     8281 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
     8341 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
     8401 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
     8461 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
     8521 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
     8581 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
     8641 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
     8701 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
     8761 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
     8821 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     8881 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     8941 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     9001 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     9061 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     9121 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     9181 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     9241 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     9301 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     9361 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     9421 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     9481 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     9541 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
     9601 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc
     9661 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
     9721 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
     9781 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
     9841 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
     9901 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
     9961 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    10021 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    10081 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    10141 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    10201 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    10261 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    10321 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    10381 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    10441 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    10501 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    10561 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    10621 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    10681 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    10741 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    10801 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    10861 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    10921 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    10981 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    11041 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    11101 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    11161 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    11221 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    11281 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    11341 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
    11401 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
    11461 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
    11521 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
    11581 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
    11641 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
    11701 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
    11761 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    11821 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
    11881 atacaaatgg acgaacggat aaacct
//
